TABLE 3.
Genetic characterization of the resistant BHS population.
| Clone | n | Phenotype | ICE-family | Ref | Other resistance genes | ||
| GAS | |||||||
| emm | MLST | res-gene | |||||
| emm11 | ST403 | erm(B) | 14 | cMLSB | Tn6003 | A | tet(M) (14), MAS (14) |
| emm58 | ST176 | erm(A) | 1 | iMLSB | ICEsp2905 | B | |
| GBS | |||||||
| Capsule | MLST | res-gene | |||||
| Ib | ST12 | erm(B) | 3 | cMLSB | ICESa2603 | C | tet(O) (3), MAS (3), lsa(E) + lnu(B) (1) |
| II | ST1 | erm(A) | 1 | iMLSB | Unknown | tet(M) | |
| II | ST12 | erm(B) | 1 | cMLSB | ICESa2603 | C | tet(O) |
| II | ST22 | erm(B) | 1 | cMLSB | Tn3872 | A | tet(M) |
| III | ST17 | erm(B) | 9 | cMLSB | ICESa2603 | C | tet(O) (9), tet(M) (2), MAS (8) |
| III | ST1434 | erm(B) | 1 | cMLSB | ICESa2603 | C | tet(O), MAS |
| III | ST19 | erm(B) | 4 | cMLSB | ICESa2603 | C | tet(O) (2), tet(M) (2), lsa(C) (2), MAS (2) |
| III | ST27 | erm(B) | 1 | cMLSB | ICESa2603 | C | tet(O) |
| III | ST1432 | erm(B) | 1 | cMLSB | Tn3872 | A | tet(M), cat(pc194) |
| III | ST335 | erm(A) | 2 | iMLSB | ICEsp2905 | B | tet(M) (2) |
| III | ST107 | erm(A) | 1 | cMLSB | ICEsp2905 | B | |
| IV | ST1433 | erm(B) | 1 | cMLSB | ICESa2603 | C | tet(O) |
| IV | ST459 | erm(A) | 6 | cMLSB | ICEsp2905 | B | tet(M) (5) |
| V | ST1 | erm(A) | 4 | iMLSB | Unknown | tet(M) (4) | |
| V | ST1 | erm(B) | 7 | cMLSB | Tn3872 | A | tet(M) (7) |
| III | ST17 | erm(T) | 1 | iMLSB | pRW35 | A | tet(M) |
| Ia | ST23 | mef | 6 | M | Phage1207.3 | A | tet(M) (6) |
| III | ST1399 | lsa(C) | 1 | L | IMESagUCN70 | D | tet(M) |
| III | ST1167 | lsa(C) | 1 | L | IMESagUCN70 | D | tet(O) |
| Ia | ST7 | ND | 1 | cMLSB | ND | ||
| SD | |||||||
| emm | MLST | res-gene | |||||
| stC74a | ST17 | erm(A) | 4 | iMLSB | ICEsp2905 | B | |
| stC74a | ST29 | erm(A) | 1 | iMLSB | ICEsp2905 | B | tet(M) |
| stG480 | ST8 | erm(A) | 2 | iMLSB | ICEsp2905 | B | |
| stG245 | ST29 | erm(A) | 1 | cMLSB | ICEsp2905 | B | |
| stG62647 | ST319 | erm(A) | 1 | iMLSB | ICEsp2905 | B | |
| stG2574 | ST23 | erm(A) | 1 | cMLSB | ICEsp2905 | B | tet(M) |
| stG485 | ST55 | erm(A) | 1 | iMLSB | ICEsp2905 | B | tet(M) + tet(O) |
| stG485 | ST17 | erm(A) | 1 | cMLSB | ICEsp2905 | B | |
| stG485 | ST29 | erm(B) | 1 | cMLSB | Unknown | ||
| stG2078 | ST473 | erm(A) | 1 | iMLSB | ICEsp2905 | B | |
| stG2078 | ST17 | erm(A) | 1 | iMLSB | ICEsp2905 | B | |
| stG2078 | ST17 | erm(B) | 1 | cMLSB | Tn6218 | * | |
| stG840 | ST269 | erm(B) | 1 | cMLSB | Tn3872 | A | tet(M) |
| stG485 | ST128 | mef(A) | 1 | M | Phage1207.3 | A | tet(M) |
| stG643 | ST12 | lsa(C) | 1 | L | ICEsp2905 | B | tet(O) |
A (Varaldo et al., 2009); B (Giovanetti et al., 2012); C (Hawkins et al., 2017), and D (Zhou et al., 2017). *Unpublished, GenBank accession HG002388. n, number of isolates; ICE, integrative conjugative element; MLST, multilocus sequence typing; res-gene, resistance gene; GAS, Streptococcus pyogenes; GBS, Streptococcus agalactiae; SD, Streptococcus dysgalactiae; MLSB, macrolide, lincosamide, streptogramin B; iMLSB, inducible MLSB-resistance; cMLSB, constitutive MLSB-resistance, M, macrolide resistance alone; L, lincosamide resistance alone; ND, no resistance gene detected. MAS, macrolide-aminoglycoside-streptothricin resistance element containing the aminoglycoside and streptothricin resistance genes aadE, aphA3, and sat4 in addition to the macrolide resistance determinant ermB.