Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 15;11:696. doi: 10.3389/fphar.2020.00696

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Wang, Bai, Zhang, Ge, Wang, Liang, Li, Gu, Li, Xu, Yang, Xu, Geng and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PUN interfered with LPS-induced activation of NF-κB and MAPK pathways. After presentative treatment with or without various concentration of PUN (0, 10, 50 μM) for 6 h, RAW264.7 cells were stimulated by100 ng/ml LPS. Then, the cells were collected and lysed for Western blot analysis (A, C–E). The relative grey level of phosphorylated protein of p65, IκBα, p-ERK/MAPK, p-38/MAPK, and JNK to total protein were quantified by using Image Lab software and compared with control group. (B) After treated with or without PUN (50 μM), RAW264.7 cells were stimulated by 100 ng/ml LPS for 1 h and then stained for p65 antibody and secondary antibody with FITC. The p65 nuclear localization was visualized using an immunofluorescence microscope.