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. 2020 May 15;13:76. doi: 10.3389/fnmol.2020.00076

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Copyright © 2020 Martel, Elseedy, Lavigne, Scapula, Ghestem, Kremer, Esclapez and Apicella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the CAV-2 construct. CAV-2 vector genomes (~32 kbp) are flanked by an inverted terminal repeat (ITR) of ~200 bp. “pChAT-eGFP-WRPE-pA” is the expression cassette that replaces the E1 region in the CAV-2 genome. “pChAT” is the promoter driving expression of choline acetylase gene, GFP is the optimized open reading frame (ORF) for green fluorescent protein (GFP) from jellyfish, WRPE is a sequence from a woodchuck hepatitis virus that increases mRNA stability and protein yield when placed 3′ to an ORF, and pA is the polyadenylation signal from human growth hormone gene (GH1). The CAV-2 E3 region is also deleted (ΔE3) to increase the packaging capacity.