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. 2020 Apr 7;295(20):6936–6945. doi: 10.1074/jbc.RA119.012213

Figure 3.

Figure 3.

HD promotes lysosomal degradation of xCT. A, immunoblot analysis of U251, T98G, and LN229 cells cultured at LD or HD with or without MG132 (20 μm) for 24 h. B, immunoblot analysis of U251, T98G, and LN229 cells cultured at LD or HD with or without BafA1 (1 μm) for 24 h. The xCT/α-tubulin ratio relative to that of vehicle treatment at LD is shown (mean ± S.D. of three independent experiments). C, immunoblot analysis of U251, T98G, and LN229 cells cultured at LD with or without BafA1 (1 μm) or Torin 1 (250 nm) for 24 h. ***, p < 0.001; **, p < 0.01; *, p < 0.05; n.s., not significant, calculated by one-way ANOVA with Tukey's post hoc test.