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. 2020 Apr 10;295(20):7060–7074. doi: 10.1074/jbc.RA119.012131

Figure 6.

Figure 6.

ZFHX3 plays a necessary role in the migration and tube formation of HUVECs involving VEGFA. A–C, tube formation of HUVECs did not increase after incubation with CM from HepG2 cells with RNAi-mediated ZFHX3 silencing under hypoxia, as indicated by cell images (A), the total length of tubes (B), and the number of nodes (C). D–F, migration of HUVECs was not significantly affected by CM from HepG2 or Huh-7 cells treated with siZFHX3 as in A–C, as indicated by migrated cells (D) and their quantification in the transwell assay (E and F). G–I, addition of VEGFA to CM partially rescued the inhibitory effect of ZFHX3 knockdown on tube formation in HUVECs, as indicated by cell images (G), the total length of tubes (H), and the number of nodes (I). Statistical analysis was based on three independent experiments (n = 3), and the value for each group in an experiment was the average of triplicate (transwell) or five fields (tube formation). Scale bar for D is 200 μm, and scale bars for A and G are 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.