Figure 1.

Workflow of the method. A, representative schematic of the active DR and the inactive DR (dDR). B, unmodified RNA is selectively cleaved by DR upstream of the target site, whereas m⁶A-modified RNA remains uncleaved. The remaining uncleaved RNAs are then quantified using RT with gene-specific reverse primer and qPCR. To control for variations in RNA input, an adjacent region on target RNA is also quantified with RT and qPCR as an internal reference. C, in the negative control sample, RNA is treated with a nonfunctional version of DR (dDR). Both m⁶A modified and unmodified RNA targets remain uncleaved and are subsequently quantified with RT and qPCR.