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. 2020 Apr 8;295(20):6992–7000. doi: 10.1074/jbc.RA120.013359

Figure 3.

Figure 3.

Validation of the method for absolute quantification of m6A fraction. GB RNA containing varied m6A fractions is used as a model system. A, PAGE showing DR cleavage fraction of the GB RNA. B, linear relationship between the input m6A fraction and the cleavage fraction of RNA by DR as quantified from the PAGE gel in A. The error bars indicate means ± S.D. for three biological replicates. C, estimated modification fraction as a function of input m6A fraction for the GB RNA. The error bars indicate means ± S.D. for at least three biological replicates.