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. 2020 Apr 8;295(20):6992–7000. doi: 10.1074/jbc.RA120.013359

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© 2020 Bujnowska et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Validation of the method for absolute quantification of m⁶A fraction. GB RNA containing varied m⁶A fractions is used as a model system. A, PAGE showing DR cleavage fraction of the GB RNA. B, linear relationship between the input m⁶A fraction and the cleavage fraction of RNA by DR as quantified from the PAGE gel in A. The error bars indicate means ± S.D. for three biological replicates. C, estimated modification fraction as a function of input m⁶A fraction for the GB RNA. The error bars indicate means ± S.D. for at least three biological replicates.