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Determination of m6A fraction of endogenous sites. A, the cleavage efficiencies (FDR) of the in vitro transcribed RNA by 60-mer DR as determined by RT and qPCR. B, determined m6A modification fractions of the 12 endogenous sites. C, determined m6A modification fractions for three endogenous targets using single and multiplexed measurements. All error bars report means ± S.D. for at least three biological replicates.
