Table 2.
Summary of ensemble molecule motility assay and parameters from single-molecule optical trapping measurements
Shown are actin filament gliding speed, lifetimes of AM-bound state, displacement of actin by individual motors, and stiffness for the WT and chimeric motor forms.
| Motors | Ensemble molecule analysis |
Single-molecule analysis |
||
|---|---|---|---|---|
| Gliding speed | Lifetimes | Displacement | Stiffness | |
| μm/s | ms | nm | pN/nm | |
| WT-S1f | 1.26 ± 0.022 | 46.4 ± 1.42 | 4.04 ± 0.41 | 1.12 ± 0.03 |
| S1f-cgmRLC | 1.56 ± 0.027 | 30.6 ± 7 | 3.99 ± 0.35 | |
| S1f-MLC2B | 1.13 ± 0.011 | 46.5 ± 2.6 | 5.63 ± 0.20 | 1.24 ± 0.09 |
| S1f-MLC2v | 0.441 ± 013 | 17 ± 0.4 | 4.34 ± 0.331 | 1 ± 0.07 |
| WT-S1s | 0.047 ± 0.001 | 282 ± 21.2 | 6.11 ± 0.16 | 0.437 ± 0.08 |
| S1s-cgmRLC | 0.458 ± 0.098 | 78.7 ± 8.2 | 6.21 ± 0.29 | 0.585 ± 0.024 |
| S1s-MLC2B | 0.075 ± 0.002 | 106 ± 9 | 1.89 ± 0.22 | 1.62 ± 0.27 |
| S1s-MLC2v | 0.052 ± 0.002 | 297 ± 11 | 4.25 ± 0.33 | 0.533 ± 0.11 |