Table 2.
PCR primers.
| Target | Sequence | Expected size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
| spvB | F:TCATACTCCAGCAGCAGACG | 587 | 50°C | This Study |
| R:AGCAGTTTTTATCGCCTGGA | ||||
| spvB-aph insa | F:GTATCAGGATAAGCACAAACAGTAAGGCGATATCCG | 1,100 | 57°C | This Study |
| R:TCATCCAATTACCTTTATTTACCAACCATAGTTTTCTTATTA | ||||
| spvC | F:CGGAAATACCATCTACAAATA | 669 | 40°C | Swamy et al., 1996 |
| R:CCCAAACCCATACTTACTCTG | ||||
| pSLT traS | F:ACCTGTCATTATTATCCTGC | 400 | 55°C | This Study |
| R:ATTATCCTGTTATTTGTCCTGC | ||||
| traS-aph insa | F:CAGGAGATAGTGTATGTTGATACTAAATGGTTTTTCATCT | 1,100 | 54°C | This Study |
| R:TATCGCCATATTATTAGATATAAATTCTCAG | ||||
| FI traS | F:TCTGCCGGAAGAATTCCTAA | 152 | 50°C | This Study |
| R:CCGTCACTAAAATTGCACCA |
a
Primers used to create λ red—targeted knockouts with aph gene cassette (Datsenko and Wanner, 2000).