Figure 8.

AAM inhibits migration of HCT-116 and LoVo cells. (A, B) Wound-healing assays of HCT-116 and LoVo cells. The cells were treated with medium alone, AAM (2.5 mg/ml), hypoxia medium, hypoxia medium with AAM (2.5 mg/ml) for 24 h. The representative images were shown in magnification of 100×. (C, D) Transwell assay was used to evaluate the effect of AAM on HCT-116 and LoVo cell migration. Migrated cells were stained and captured by microscope (50×); cell numbers are compared between groups. (*P < 0.05, **P < 0.01, ***P < 0.001).