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. 2020 May 20;24:100604. doi: 10.1016/j.ymgmr.2020.100604

Fig. 3.

Fig. 3

Immunohistologic analysis of IDUA expression. A-C, IDUA immunoreactivity (−ir) was abundant in livers from AAV9- (A) and AAVrh10 (B)-treated mice, while labeling was absent in liver from an untreated MPS I mouse (C). Scale bar: 50 μm. D-F, A representative image of double-labeling for IDUA (D) and the neuronal marker NeuN (E), showing lack of colocalization (F) in the cortex of an AAV9-treated mouse. Scale bar: 50 μm. G-I, A representative image of double-labeling for IDUA (G) and the astrocyte marker GFAP (H), showing lack of colocalization (I) in hippocampus of an AAVrh10-treated mouse. Arrows indicate an example of a blood vessel with closely associated IDUA- and GFAP-ir. Scale bar: 50 μm. J-L, Double-labeling for IDUA (J) and the endothelial marker CD31 (K) in cortex of an AAV9-treated mouse reveals apparent IDUA-positive cellular profiles that are closely associated with a CD31-ir blood vessel. White puncta correspond to autofluorescent structures. Scale bar: 10 μm.