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. 2020 Apr 28;39:101006. doi: 10.1016/j.molmet.2020.101006

Figure 7.

Figure 7

DDR1 deficient mice have decreased adipose fibrosis, and DDR1 is upregulated during fibrogenic differentiation of C3H10T1/2 cells in vitro. (A) sFat from Ddr1+/+ and Ddr1−/− mice fed HFD was stained with Picrosirius Red (PSR) to visualize collagen accumulation. (B) Peri-adipocyte collagen was quantified (n = 6). (C) Collagen mRNA expression in sFat obtained from Ddr1+/+ and Ddr1−/− mice after 12 weeks on HFD (n = 4). (D-E) DDR1 and MRTF-A protein expression in sFat obtained from Ddr1+/+ and Ddr1−/− mice after 12 weeks on HFD (n = 6). (F-G) DDR1b was transfected in C3H10T1/2 cells (without differentiation) and MRTF-A expression was assessed by immunoblot (n = 3). (H-L) C3H10T1/2 cells were treated with TGFβ to stimulate fibrocyte differentiation, and expression of DDR1 and fibrosis markers was assessed by immunoblot (H), and quantified (I-L) (n = 3). Statistical analysis was performed using student's t-test (B, C, E, and G), and 1-way ANOVA with Bonferroni post-hoc test (I-L). The values are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.