Skip to main content
. 2020 Apr 24;39:101004. doi: 10.1016/j.molmet.2020.101004

Figure 3.

Figure 3

Ex vivo studies comparing native calcium current in cultured hypothalamic neurons from GHSR-A203E-null, wild-type, and GHSR-A203E mice. (A) Representative traces of barium currents (IBa) and (B) mean IBa current values from cultured hypothalamic neurons from GHSR-A203E-null (n = 16), wild-type (including a combination of wild-type littermates of both the GHSR-A203E-null and GHSR-A203E lines; n = 10), and GHSR-A203E (n = 25) mice. (C) Normalized representative traces of IBa currents and (D) mean IBa current percentage inhibition by ghrelin of hypothalamic neurons from GHSR-A203E-null (n = 5), wild-type (n = 4), and GHSR-A203E mice (n = 11) in the absence and presence of ghrelin (500 nM). Data were analyzed by Kruskal–Wallis ANOVA with Dunn's post hoc analysis. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, and n.s. = non-significant.