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. 2020 Apr 24;39:101004. doi: 10.1016/j.molmet.2020.101004

Figure 4.

Figure 4

Ex vivo studies comparing electrophysiological properties of arcuate NPY neurons from wild-type vs GHSR-A203E littermates. (A) Representative NPY-GFP neurons from a wild-type mouse on an NPY-GFP background under (i) bright-field illumination and (ii) FITC (GFP) illumination. Complete dialysis of Alexa Fluor 350 from the intracellular pipette is shown in (iii). A merged image of the targeted NPY neuron is shown in (iv). Arrow indicates the targeted cell. Scale bar = 50 μm. (B) Representative NPY-GFP neuron from a GHSR-A203E mouse on an NPY-GFP background under (i) bright-field illumination and (ii) FITC (GFP) illumination. Complete dialysis of Alexa Fluor 594 from the intracellular pipette is shown in (iii). A merged image of the targeted NPY neuron is shown in (iv). Arrow indicates the targeted cell. Scale bars = 50 μm. (C and D) Representative current-clamp recordings of NPY neurons from (C) wild-type and (D) GHSR-A203E mice demonstrating resting membrane potentials and depolarization upon addition of ghrelin (100 nM). (E) Change in membrane potential in NPY neurons from wild-type or GHSR-A203E mice in response to ghrelin (100 nM) or vehicle (ACSF). Data were analyzed by 2-way ANOVA followed by a Tukey post hoc analysis. ∗∗P < 0.01. (F–G) Linear regression analyses of current–voltage (I–V) plots of (F) wild-type (G) and GHSR-A203E mice.