TABLE 2.
Kinetic parameters of the wild-type XacXynB and the mutants K166D and D167G on 4-nitrophenyl β-D-xylopyranoside.
KM (mM) | kcat (min–1) | kcat/KM (min–1 mM–1) | |
WT | 1.93 ± 0.25 | 266.0 ± 8.5 | 137.8 |
K166D | 4.06 ± 0.39 | 219.2 ± 6.8 | 54.0 |
D167G | 5.79 ± 0.35 | 281.0 ± 6.2 | 48.5 |