Table 1.
Sequence and reason of selection of the differentially expressed miRNAs used for validation
| SN | miRNA | miRNA primer name | Primer Sequence (5′-3′) | Comments on the specific miRNAa |
|---|---|---|---|---|
| 1 | bta-miR-9-5p | BbuMir16-F | ucuuugguuaucuagcug | To compare Br-Mu with Ctrl-Mu |
| 2 | bta-miR-677 | BbuMir19-F | cucacugaugagcagcuu | |
| 3 | bta-miR-331-3p | BbuMir18-F | gccccugggccuauccua | To compare JD-Mu with Ctrl-Mu |
| 4 | bta-miR-2440 | BbuMir21-F | ugcagugaugagacccug | |
| 5 | Endogenous Control | BbuRNU6 | acgcaaauucgugaagcguu |
aBr-Mu: Brucella infected buffaloes of Murrah breed
JD-Mu: Johne’s Disease infected buffaloes of Murrah breed
Ctrl-Mu: Healthy buffaloes of Murrah breed