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. 2020 May 22;11(5):390. doi: 10.1038/s41419-020-2544-7

Fig. 3. Glucose induces a metabolic switch from FAO to glycolysis in primary tubular epithelial cells (PTCs).

Fig. 3

a Representative images of PTCs incubated with 30 mmol/l of glucose for 24 h with Oil Red O staining. Scale bars, 25 μm. b Quantification of Oil Red O-positive area in PTCs incubated with 30 mmol/l of glucose for 24 h. n = 3 experiments. **p < 0.01 compared to control. c Relative mRNA levels of genes related to FAO (Pparα, Cpt1α, and Acadl) in PTCs incubated with 30 mmol/l of glucose for 6 h. *p < 0.05 compared with control; **p < 0.01 compared with control. d Western blot analysis of proteins related to FAO and relative protein expression levels in control and HG-treated PTCs. **p < 0.01 compared with control. e Glucose decline in medium of PTCs incubated with 30 mmol/l of glucose for various time periods, as indicated. **p < 0.01 compared with control. f Relative mRNA levels of genes related to glucose utilization (Hk2, Ldh, and Pdk1) in PTCs incubated with 30 mmol/l of glucose for 6 h. *p < 0.05 compared with control; **p < 0.01 compared with control. g Western blot analysis of proteins related to glucose utilization and relative protein expression levels in control and HG-treated PTCs. *p < 0.05 compared with control. **p < 0.01 compared with control. h Quantification of lactate in culture medium of PTCs incubated with 30 mmol/l of glucose for various time periods, as indicated. **p < 0.01 compared with control.