Figure 6.

Investigation of the Effect of Protein Fusion on the Affinity of HiBiT-LgBiT Complementation

(A–C) HEK293 cells expressing genome-edited HiBiT/CXCR4 (black symbols) or wild-type HEK293 cells (white squares) (A), membranes from HEK293 cells expressing genome-edited HiBiT/CXCR4 (black symbols) and wild-type HEK293 cells (white squares) (B), or purified HiBiT control protein (black symbols) were incubated with increasing concentrations of LgBiT in the absence (black circles) or presence (black squares) of AMD3100 (10 μM) (C).

(D) HEK293 cells expressing genome-edited HiBiT/β₂-adrenoceptor (downward triangles) or wild-type HEK293 cells (white squares) were incubated with increasing concentrations of purified LgBiT. Points are mean ± SEM and are representative of five (C and D), six (B), or seven (A) experiments performed in triplicate.