REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
AMD3100	Selleckchem	Cat # S8030
Covine serum albumin (Protease-free)	Sigma Aldrich	Cat# 03117332001
CXCL11	Preprotech	Cat# 300-46
CXCL12	Preprotech	Cat# 300-28A
CXCL12-AF647	Almac	Cat# CAF-11
N,N'-Dicyclohexylcarbamimidothioic acid (5,6-dihydro-6,6-dimethylimidazo[2,1-b]thiazol-3-yl)methyl ester dihydrochloride (IT1t)	Tocris	Cat# 4596
Dulbecco’s Modified Eagle’s Medium	Sigma Aldrich	Cat# D6429
Fetal Bovine Serum	Sigma Aldrich	Cat# F2442
Fugene HD	Promega (Wisconsin, USA)	Cat# E2311
Geneticin™ (G418)	ThermoFisher	Cat# 10131035
HiBiT-Halotag, control peptide	Promega (Wisconsin, USA)	Cat# N3010
Isoprenaline hydrochloride	Sigma Aldrich	Cat# I6504
Phosphate Buffered Saline (PBS)	Sigma Aldrich	Cat# D8537
Poly-D-Lysine hydrobromide	Sigma Aldrich	Cat# P6407
(±)-propranolol hydrochloride	Sigma Aldrich	Cat# P0884
Purified LgBiT	Promega (Wisconsin, USA)	Cat# N401A
Purified full length NLuc	Promega (Wisconsin, USA)	Gift from Matt Robers (Promega)
Puromycin dihydrochloride from Streptomyces alboniger	Sigma Aldrich	Cat# P8833
Saponin	Sigma Aldrich	Cat# 84510
Xanthine amine congener (XAC)	Sigma Aldrich	Cat# X103
Critical Commercial Assays
Maxima First Strand cDNA Synthesis Kit	Thermo Fisher Scientific	Cat# K1641
Nano-Glo luciferase assay system (Furimazine)	Promega (Wisconsin, USA)	Cat# N1130
Pierce™ bicinchoninic acid protein assay kit	Thermo Fisher Scientific	Cat# 23225
PowerUp SYBR Green Master Mix	Thermo Fisher Scientific	Cat# A25742
ReliaPrep™ RNA extraction kit	Promega Corporation (Wisconsin,USA)	Cat# Z6010
Hs_B2M_1_SQ QuantiTect Primer Assay	Qiagen	Cat# QT00088935
Experimental Models: Cell Lines
Human HEK293FT cells (female)	Life Technologies	Cat# R70007
Human HeLa cells (female)	Laboratory of Stephen Briddon (University of Nottingham)	Rose et al., 2012
Oligonucleotides
Oligonucleotides for sgRNA construction, see Table S1	Sigma Aldrich	Custom Synthesis
Oligonucleotides for PCR amplification, see Table S1	Sigma Aldrich	Custom Synthesis
Oligonucleotides for site directed mutagenesis, see Method Details - Molecular Biology	Sigma Aldrich	Custom Synthesis
Single stranded oligonucleotide for ADRB2 homology directed repair template, see Table S2	Integrated DNA Technologies	Custom Synthesis
Recombinant DNA
β-arrestin2-SmBiT	This manuscript	Custom synthesis
β-arrestin2-Halotag	Tiulpakov et al., 2016	Custom synthesis
cDNA encoding NSGSSGGGGSGGGGSSG-LgBiT for sub-cloning	GeneArt (Thermo Fisher Scientific)	Custom synthesis
CXCR4-LgBiT	This manuscript	Custom synthesis
HiBiT-ACKR3	This manuscript	Custom synthesis
HiBiT-CXCR4	This manuscript	Custom synthesis
Homology directed repair templates, see Table S2	GeneArt (Thermo Fisher Scientific)	Custom synthesis
NanoLuc-ACRK3	This manuscript	Custom synthesis
NanoLuc-CXCR4	This manuscript	Custom synthesis
pSpCas9(BB)-2A-Puro (PX459) V2.0	Addgene Plasmid	Cat # 62988; RRID: Addgene_62988
Software and Algorithms
GraphPad Prism 7.02	GraphPad Software, La Jolla California USA	https://www.graphpad.com/ scientific-software/prism/
Other
35 mm dish containing a high tolerance 1.5 μm coverslip	MatTek	Cat# P35G-0.170-14-C
BamHI-HF Restriction enzyme	New England Biolabs (UK)	Cat# R3136
Kpn-HF Restriction enzyme	New England Biolabs (UK)	Cat# R3142
PHERAStar FS plate reader	BMGLabTech	PHERAStar FS plate reader
Olympus LV200 wide field inverted microscope	Olympus	Olympus LV200 wide field inverted microscope
Q5® High-Fidelity DNA Polymerase	New England Biolabs (UK)	Cat# M0491
XbaI Restriction enzyme	New England Biolabs (UK)	Cat# R0145
XhoI Restriction enzyme	New England Biolabs (UK)	Cat# R0146
White 96-well plates	Greiner Bio-One	Cat# 655089