Fig. 2.

Detection of total IgM-secreting 15 and 30 days after the anal administration of the antigens. To determine the amount of total IgM-secreting cells in (A) spleen and (B) kidney, ELISPOT plates were coated with an anti-trout IgM mAb (clone 4C10) and developed using the same mAb biotinylated. Results are shown as mean number of IgM-secreting cells per 5 × 10⁴ cells +SD (n = 7). Representative wells for each condition are shown. Asterisks denote significantly different values among groups as indicated (*p ≤ 0.05 and ***p ≤ 0.005).