Fig. 7.

Transcription of genes related to B and T cell activity in spleen (A), kidney (B) and gut (C) 15 days after the anal administration of antigens. Total RNA was extracted from the tissues obtained from vaccinated fish sacrificed at day 15 post-immunization and used to determine the levels of transcription of different genes related to B and T cell activity by real time PCR. Results are shown as the gene expression relative to the expression of endogenous control EF-1α (mean +SD, n = 7). Asterisks denote significantly different values among groups as indicated (*p ≤ 0.05 and **p ≤ 0.01).