DGKE mutations. (a) Predicted protein model of diacylglycerol kinase epsilon (DGKE) demonstrating positions of rare genetic variants. DGKE protein model demonstrating positions of rare genetic variants. Phyre2 was used to create a predicted protein model of DGKE. Amino acid substitutions are shown by red spheres. Transmembrane domain (amino acids 22–42) is shown in blue, cysteine-rich domain 1 (amino acids 60–109) in dark green, cysteine-rich domain 2 (amino acids 125–178) in light green, kinase catalytic domain (amino acids 219–350) in pink, and kinase accessory domain (amino acids 369–524) in orange. Amino acids L431 and L438, thought to be important for diacylglycerol specificity, are shown in blue spheres. (b) Schematic representation of (i) DGKE protein and (ii) DGKE DNA sequence showing relative positions of variants.