Figure 1.

Distinction of Melanoma Stromal Populations with Single-Cell RNA-Seq

(A) Overview of experimental and sequencing workflow.

(B) t-Distributed Stochastic Neighbor Embedding (tSNE) visualization of all cells sequenced with each cell color coded for cell type (left), site of origin (center), and time (right).

(C) Expression of marker genes for each cell type. n = 32 mice.

cDC1/2, conventional dendritic cell; DC LN, lymph node dendritic cell; Endo lymph, lymphatic endothelial cell; endo LN, lymph node endothelium; Endo tumor, tumor endothelial cells; fibroblast LN, lymph node fibroblast; MAIT, mucosal-associated invariant T cell; migDC, migratory DC; NK, natural killer; pDC, plasmacytoid DC.