Figure 2.

Lagging Strand PCNA Retention Facilitates Cohesion Establishment

(A) Cells of the indicated genotypes were synchronized in G1 and treated with auxin for 2 h to deplete Cdc9 before release into nocodazole-containing medium. Sister chromatid cohesion was assessed at the GFP-marked URA3 locus. Means ± SE from three independent experiments are shown.

(B) As in (A) but Smc3 acetylation was quantified relative to total Smc3 levels. Means ± SE from three independent experiments are shown.

See Figure S3 for further analyses on the role of PCNA in cohesion establishment.