Figure 4.

Pol ε Interaction Is Dispensable for Ctf18-RFC Function

(A) Schematic of the Ctf18 interaction with the Pol ε large subunit Pol2.

(B) Cells were synchronized in G1 and released into HU-containing medium for an early S phase arrest. Ctf18 enrichment close to an early (ARS606 and 607) and a late firing (ARS609) replication origin were quantified by real-time PCR. Means ± SE from three independent experiments are shown.

(C) Cells were synchronized in G1 and released into nocodazole-containing medium. Sister chromatid cohesion was assessed at the GFP-marked URA3 locus. Means ± SE from three independent experiments are shown.

(D) As in (C), but Smc3 acetylation was quantified relative to total Smc3 levels. Means ± SE from three independent experiments are shown.

(E) 10-fold serial dilutions of the indicated strains were spotted on Yeast extract Peptone Dextrose (YPD) agar plates without or containing 100 mM HU.

See Figure S5 for further characterization of Pol2^EDD and Dcc1^KRR.