REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Smc3Ac	Gift from Shirahige Lab	N/A
Mouse monoclonal anti-Smc3 (361 F3C6, budding yeast)	Gift from Shirahige Lab	N/A
Mouse monoclonal anti-PCNA (5E6/2)	Cell Services Science Technology Platform, The Francis Crick Institute	N/A
Rabbit polyclonal anti-PCNA (Ab871, for eSPAN)	Gift from Zhang Lab	N/A
Rabbit polyclonal anti-V5	Abcam	ab15828
Rabbit polyclonal anti-HA	Abcam	ab9110
Rabbit polyclonal anti-Rad53	Abcam	ab104232
Mouse monoclonal anti-HA (12CA5)	Cell Services Science Technology Platform, The Francis Crick Institute	N/A
Mouse monoclonal anti-FLAG (M2)	Merck	Cat# F3165
Mouse monoclonal anti- α-tubulin (TAT-1)	Cell Services Science Technology Platform, The Francis Crick Institute	N/A
Mouse monoclonal anti-HIS (6G2A9)	Genscript	Cat# A00186
Anti-mouse IgG (HRP-conjugated)	GE Healthcare	Cat# NA931
Anti-rabbit IgG (HRP-conjugated)	GE Healthcare	Cat# NA934
Chemicals, Peptides, and Recombinant Proteins
α-factor	Peptide Chemistry Science Technology Platform, The Francis Crick institute	N/A
Nocodazole	Merck	Cat# M1404
Hydroxyurea	Merck	Cat# H8627
Indole-3-acetic acid (IAA)	Merck	Cat# I3750
5-Bromo-2’-Deoxyuridine	Merck	Cat# B5002
G418	Merck	Cat# G8618
Formaldehyde solution	Merck	Cat# 252549
Paraformaldehyde	Merck	Cat# P6148
Phenylmethylsulfonyl fluoride (PMSF)	Merck	Cat# P7626
Pefabloc SC	Roche	Cat# 11 429 876 001
cOmplete EDTA-Free Protease Inhibitor Cocktail	Merck	Cat# 04693132001
Benzamidine	Merck	Cat# 12072
Bacitracin	Merck	Cat# B0125
Proteinase K	ThermoFisher	Cat# EO0491
Leupeptin	Generon	Cat# 51867.02
Benzonase Nuclease	Merck	Cat# E1014
RNase A	Merck	Cat# 10109169001
Protein Assay Dye	Bio-Rad	Cat# 5000006
Propidium iodide solution	Merck	Cat# P4864
GelRed Nucleic Acid Gel Stain	Biotium	Cat# 41003-1
ApaI	New England Biolabs	Cat# R0114S
APE1	New England Biolabs	Cat# M0282S
BsaBI	New England Biolabs	Cat# R0537L
tRNA (Bacterial, MRE600)	Merck	Cat# 10109541001
Chelex 100 Resin	Bio-rad	Cat# 1421253
Uracil DNA Glycosylase	ThermoFisher	Cat# EN0361
Recombinant Terminal Deoxynucleotidyl Transferase	ThermoFisher	Cat#10533065
Biotin-11-dXTP Tetralithium Salt	Affymetrix	Cat# 79015
SSPE	VWR International	Cat# J61214.K2
Imidazole	Merck	Cat# 68268
TCEP	Fluorochem Limited	Cat# M02624
SIGMAFAST™ Protease Inhibitor Tablets	Merck	Cat# S8820
Acetyl CoA	Merck	Cat# A2181
Critical Commercial Assays
GeneChip™ S. cerevisiae Tiling 1.0R Array	ThermoFisher	Cat# 900645
HisTrap™ Fast Flow Crude	Merck	Cat# GE29-0486-31
Protein G Sepharose Beads Fast Flow	GE	Cat# 17-0618-01
Protein A Dynabeads	ThermoFisher	Cat# 10002D
ECL Prime Western Blotting Detection Regent	GE Healthcare	Cat# RPN2232
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	Cat# E05545
InFusion HD cloning kit	Clontech Laboratories	Cat# 639634
CloneAmp HiFi PCR Premix	Clontech Laboratories	Cat# 639298
Whole Genome Amplification 2 Kit	Merck	Cat# WGA2
Agencourt AMPure XP Beads	Beckman-Coulter	Cat# A63881
Accel-NGS 1S Plus DNA Library Kit	Swift Biosciences	Cat# 10096
Accel-NGS 1S Unique Dual Indexing Kit	Swift Biosciences	Cat# 19096
ExoSAP-IT Express PCR Product Cleanup Reagent	VWR International	Cat# 75001
PowerUp SYBR Green Master Mix	ThermoFisher	Cat# A25742
Experimental Models: Organisms/Strains
All Saccharomyces cerevisiae strains used in this study are listed in Table S1	Lab stock and this study	N/A
Escherichia coli DH5α competent cells	New England Biolabs	Cat# C2987U
Oligonucleotides
All oligonucleotides used for qPCR are listed in Table S2	N/A	N/A
Software and Algorithms
Snapgene v2.6	GSL Biotech	N/A
FlowJo v10.1	FlowJo	N/A
ImageQuant TL v8.1	GE Healthcare	N/A
ImageJ v1.50c	NIH, USA	N/A
Deposited Data
ChIP chip and eSPAN sequencing data	GEO Accession Number GSE138056	N/A
Unprocessed gel images	https://data.mendeley.com/datasets/sh2wjmfvfh/draft?a=27586cf4-2026-483f-bcf0-2446ff5ef228	N/A