REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-AQP4 antibody	Abcam	Cat#:ab46182; RRID: AB_955676
Mouse monoclonal anti-AQP4 antibody	Abcam	Cat#:ab9512; RRID: AB_307299
Rabbit anti-AQP4 antibody	Abcam	Cat#:ab128906; RRID: AB_11143780
Chicken anti-mouse IgG-HRP antibody	Santa Cruz	Cat#:sc-2954; RRID: AB_639239
Donkey anti-rabbit IgG-HRP antibody	Santa Cruz	Cat#:sc-2313; RRID: AB_641181
Rabbit anti-Foxo3 IgG antibody	Abcam	Cat#:ab23683; RRID: AB_732424
Mouse anti-RECA-1 antibody	Abcam	Cat#:ab9774; RRID: AB_296613
Goat anti-rabbit IgG FITC antibody	Merck	Cat#:F0382; RRID: AB_259384
Goat anti-chicken IgY Alexafluor 488 antibody	Abcam	Cat#:ab150169; RRID: AB_2636803
Goat anti-mouse IgG Alexafluor 633 antibody	ThermoFisher	Cat#:A-21050; RRID: AB_141431
Chicken anti-albumin antibody	Abcam	Cat#:ab106582; RRID: AB_10888110
Rabbit polyclonal anti-laminin antibody	Sigma	Cat#:L9393; RRID: AB_477163
Mouse anti β-actin antibody	Sigma-Aldrich	Cat#:A2228; RRID: AB_476697
Rabbit anti-calmodulin antibody	Cell Signaling	Cat#:D1F7J; RRID: AB_2799090
Rabbit anti-lamin B1 antibody	Cell Signaling	Cat#:D4Q4Z; RRID: AB_2650517
Rabbit anti-alpha tubulin antibody	Cell Signaling	Cat#:2144; RRID: AB_2210548
Mouse anti-His tag antibody	Takara Bio	Cat#:631212; RRID: AB_2721905
Bacterial and Virus Strains
E.coli strain DH5α	Thermo Fisher	18265017
One Shot BL21 (DE3) E. coli	Thermofisher	C600003
One Shot TOP10 E. coli	Thermofisher	C404010
Chemicals, Peptides, and Recombinant Proteins
Trifluoperazine dihydrochloride	Sigma-Aldrich	T8516
W-7 hydrochloride	Sigma-Aldrich	681629
L-741,626	Sigma-Aldrich	L135
Terazosin hydrochloride	Sigma-Aldrich	T4680
H89	Tocris	2910
Gö6983	Tocris	2285/1
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)	Sigma	42773
2-Oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG)	Sigma	76559
Cholesterol	Sigma	C8667
5(6)-Carboxyfluorescein	Sigma	21877
Alexa Fluor 488 maleimide	ThermoFisher	A10254
NH4Cl (15N, 99%)	Cambridge Isotopes	NLM-467-5; CAS# 39466-62-1
D-glucose (1-13C, 98-99%)	Cambridge Isotopes	CLM-1396-5; CAS# 110187-42-3
Glutathione Sepharose 4B	GE Healthcare	17-075601
PreScission protease	GE Healthcare	27-084301
Phos-Tag acrylamide	NARD Institute	AAL-107
2,2-dimethyl-2-silapentane-5-sulfonate	Cambridge Isotopes	DLM-32-10; CAS# 2039-96-5
D2O (D, 99.96%)	Cambridge Isotopes	DLM-6-1000; CAS# 7789-20-0
Amicon Ultra-15 centrifugal filter unit	Millipore-Sigma	UFC900308
M9 minimal media salts	Millipore-Sigma	M9956-500ML
Recombinant human AQP4 (UniProtID P55087)	Öberg et al., 2011	N/A
Recombinant human AQP4- Δ256	This paper	N/A
Recombinant human AQP4-F258/262/268A	This paper	N/A
Recombinant human AQP4-S276E	This paper	N/A
Recombinant GST-recAQP4ct (containing human AQP4 residues 254-323)	This paper	N/A
Recombinant human calmodulin S17C (UniProtID P0DP23)	O’Connell et al., 2010	N/A
Recombinant chicken calmodulin	Nakashima et al., 1996	N/A
Bovine heart cAMP-dependent protein kinase (PKAc)	Hofmann et al., 1975	N/A
EZ-Link Sulfo-NHS-SS-biotin, cell impermeable biotinylation reagent	ThermoFisher	21331
Calcein-AM	ThermoFisher	C3100MP
Critical Commercial Assays
RNeasy Plus Mini Kit	QIAGEN	74134
QIAquick PCR Purification Kit	QIAGEN	28104
Pierce BCA Protein Assay Kit	ThermoFisher	23225
ELISA-based assay kit for PKA activity	Abcam	ab139435
ELISA-based assay kit for Akt activity	Abcam	ab139436
Experimental Models: Cell Lines
Rat primary cortical astrocytes	GIBCO	N7745100
Human cerebral cortex primary astrocytes	Sciencell	1800
HEK293	ATCC	CRL-1573
Experimental Models: Organisms/Strains
Rats/Sprague-Dawley	Charles River UK	CD IGS
Pichia pastoris strain X-33	Thermo Fisher	C18000
Oligonucleotides
Primer recAQP4ct: NT forward 5′-GCGCGGATCCCCAGATGT TGAATTCAAACG	This study	N/A
Primer recAQP4ct: NT reverse 5′-CCATCTGGAGAGGTATT GTCTTCA	This study	N/A
Primer recAQP4ct: CT forward 5′-CAATCTGGAGAGGTATT GTCTTCAGTATAAGCGGCCGCGCGC	This study	N/A
Primer recAQP4ct: CT reverse 5′- GCGCGCGGCCGCTTAT ACTGAAGACAATACCTCTCCAGATTG	This study	N/A
Recombinant DNA
pGFP-C-shLenti-AQP4	Origene	Cat no. TL709442, Locus ID: 25293
pGFP-C-shLenti-Control	Origene	Cat no. TR30021
pDEST47-hAQP4-His6	This study	N/A
pDEST47-hAQP4-F258A-His6	This study	N/A
pDEST47-hAQP4-F262A-His6	This study	N/A
pDEST47-hAQP4-F266A-His6	This study	N/A
pDEST47-hAQP4-F258/262/266A-His6	This study	N/A
pGEX-6P1	GE Healthcare	27-1542-01
Software and Algorithms
NMRPipe	NIST IBBR	https://www.ibbr.umd.edu/nmrpipe/
NMRViewJ	NMRFx	http://nmrfx.org/nmrfx/nmrviewj