Figure 5.
Catalytic center, incorporation and implications on inhibition of nucleotide analog inhibitors
(A) Close view of the catalytic center in the RDV-MP incorporated pre-translocated complex. Interactions are shown as dashed lines and measured in Å.
(B) The base pair of cytosine and guanosine at +1 position and the coordinating residues under unambiguous experimental cryo-EM map signal.
(C) Favipiravir-DP is shown as the stick in olive, aligned on the guanosine at +1 position.
(D) The base pair of uracil and incorporated RDV-MP at −1 position under experimental cryo-EM map signal. The 2D chemical structure of the incorporated RDV-MP is provided.
(E) Structural demonstration of the delayed chain termination hypothesis of RDV. RDV (in gray) is aligned onto the product strand (in purple) to demonstrate their incorporated state at −4 position.