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Representative SEM micrographs and corresponding fluorescence images of DAPI/phalloidin stained ARPE-19 cells cultured on PET membranes for 1 month (A–C) and 3 months (D–F). Cells populate the membrane with better coverage and more apparent phalloidin staining at 3 months culture. Microvilli (arrows) phenotypical of RPE cells are apparent by 1 month (n = 3).
