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The membrane does not allow invasion of cells through its architecture. H&E staining of cells cultured on the membrane for 1 week (A) up to 3 months (B) show the desired lack of cell invasion. The SEM micrograph (C) exhibits a lack of cells on the basal side of the membrane that had been seeded with ARPE-19 cells on its apical side and cultured for 3 months (n = 4).
