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. Author manuscript; available in PMC: 2021 Apr 16.
Published in final edited form as: Mol Cell. 2020 Apr 16;78(2):289–302.e6. doi: 10.1016/j.molcel.2020.01.026

Figure 2. The Cluster Assistance Enhances Processing of Other Mammalian miRNA Hairpins.

Figure 2.

(A) The human miRNA hairpins used in the over-expression experiments in (C). Otherwise, this panel is as in Figure 1B.

(B) Schematics of the expression cassettes used in (C). Otherwise, this panel is as in Figure 1C.

(C) Northern-blot analysis of miRNA accumulation from the indicated expression cassettes.

(D) Quantification of (C) and its biological replicate (circles and triangles, respectively). For each lane, mature miRNA levels were first normalized to that of miR-1 and then to the levels from individually expressed miR-181b-1 or miR-125a (mean of two replicates). Statistically significant change of miR-181b-1 level compared with the level of individually expressed miR-181b-1 is indicated (***p < 0.001, unpaired two-tailed t test).

(E) The human miRNA hairpins used in the over-expression experiments in (G). Otherwise, this panel is as in Figure 1B.

(F) Schematics of the expression cassettes used in (G). Otherwise, this panel is as in Figure 1C.

(G) Northern-blot analysis of miRNA accumulation from the indicated expression cassettes.

(H) Quantification of (G) and its biological replicate (circles and triangles, respectively). For each lane, mature miRNA levels were first normalized to that of miR-1 and then to the levels from individually expressed miR-374b or miR-421 (mean of two replicates). Statistically significant changes of miR-374b and miR-421 levels compared with the levels of individually expressed miRNAs are indicated (*p < 0.05, unpaired two-tailed t test).