Table 1. Primers for amplification of target genes for detection of disease-causing camel pathogens.
Primer name | 5’to 3’ sequence | Target
organism |
Target gene | Product
size (bp) |
References for primers |
---|---|---|---|---|---|
IS1111F
IS1111F |
GTA ATA TCC TTG GGC GTT GAC G
ATC TAC GCA TTT CAC CGC TAC AC |
C. burnetii |
Coxiella 16S
rRNA |
242 | ( Doosti et al., 2014) |
AnaplasmaJV
F
AnaplasmaJV R |
CGGTGGAGCATGTGGTTTAATTC
CGRCGTTGCAACCTATTGTAGTC |
Anaplasma spp. |
Anaplasma
16S rRNA |
300 | ( Mwamuye et al., 2017) |
EhrlichiaJV F
EhrlichiaJV R |
GCAACCCTCATCCTTAGTTACCA
TGTTACGACTTCACCCTAGTCAC |
Ehrlichia spp. |
Ehrlichia 16S
rRNA |
300 | ( Mwamuye et al., 2017) |
EHR16SD
1492R |
GGTACCYACAGAAGAAGTCC
GGTTACCTTGTTACGACTT |
Ehrlichia & Anaplasma spp. | 16S rRNA | 1000 | (
Parola
et al., 2000;
Reysenbach et al., 1992) |
RLB F
RLB R |
GAGGTAGTGACAAGAAATAACAATA
TCTTCGATCCCCTAACTTTC |
Theileria &
Babesia spp. |
Theileria &
Babesia 18S rRNA |
450 | |
CMLVC18LF
CMLVC18LR |
GCGTTAACGCGACGTCGTG
GATCGGAGATATCATACTTTACTTTAG |
Camel pox virus | C18L gene | 243 | ( Balamurugan et al., 2009) |
B. arbutus F
B. melitensis F Brucella IS711T R |
GCGCTCAGGCTGCCGACGCAA
GCGGCTTTTCTATCACGGTATTC GGGTAAAGCGTCGCCAGAAG |
Brucella spp | IS711 | ( Probert et al., 2004) | |
ITS1_CF
ITS1_BR |
CCGGAAGTTCACCGATATTG
TTGCTGCGTTCTTCAAC- GAA |
Trypanosoma
spp. |
ITS1 | 250-720 | ( Njiru et al., 2005) |
ILO 7957F
ILO 8091R |
GCCACCACGGCGAAAGAC
TAATCAGTGTGGTGTGC |
T. evansi | RoTat 1.2 | 488 | ( Urakawa et al., 2001) |