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. 2020 May 20;2:164. Originally published 2019 Nov 5. [Version 2] doi: 10.12688/aasopenres.13021.2

Table 1. Primers for amplification of target genes for detection of disease-causing camel pathogens.

Primers sequences were sent to inqaba biotec™ (Muckleneuk, Pretoria, South Africa) for synthesis.

Primer name 5’to 3’ sequence Target
organism
Target gene Product
size (bp)
References for primers
IS1111F
IS1111F
GTA ATA TCC TTG GGC GTT GAC G
ATC TAC GCA TTT CAC CGC TAC AC
C. burnetii Coxiella 16S
rRNA
242 ( Doosti et al., 2014)
AnaplasmaJV F
AnaplasmaJV R
CGGTGGAGCATGTGGTTTAATTC
CGRCGTTGCAACCTATTGTAGTC
Anaplasma spp. Anaplasma
16S rRNA
300 ( Mwamuye et al., 2017)
EhrlichiaJV F
EhrlichiaJV R
GCAACCCTCATCCTTAGTTACCA
TGTTACGACTTCACCCTAGTCAC
Ehrlichia spp. Ehrlichia 16S
rRNA
300 ( Mwamuye et al., 2017)
EHR16SD
1492R
GGTACCYACAGAAGAAGTCC
GGTTACCTTGTTACGACTT
Ehrlichia & Anaplasma spp. 16S rRNA 1000 ( Parola et al., 2000;
Reysenbach et al., 1992)
RLB F
RLB R
GAGGTAGTGACAAGAAATAACAATA
TCTTCGATCCCCTAACTTTC
Theileria &
Babesia spp.
Theileria &
Babesia 18S
rRNA
450
CMLVC18LF
CMLVC18LR
GCGTTAACGCGACGTCGTG
GATCGGAGATATCATACTTTACTTTAG
Camel pox virus C18L gene 243 ( Balamurugan et al., 2009)
B. arbutus F
B. melitensis F
Brucella IS711T R
GCGCTCAGGCTGCCGACGCAA
GCGGCTTTTCTATCACGGTATTC
GGGTAAAGCGTCGCCAGAAG
Brucella spp IS711 ( Probert et al., 2004)
ITS1_CF
ITS1_BR
CCGGAAGTTCACCGATATTG
TTGCTGCGTTCTTCAAC- GAA
Trypanosoma
spp.
ITS1 250-720 ( Njiru et al., 2005)
ILO 7957F
ILO 8091R
GCCACCACGGCGAAAGAC
TAATCAGTGTGGTGTGC
T. evansi RoTat 1.2 488 ( Urakawa et al., 2001)