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. 2020 May 22;13:93. doi: 10.1186/s13068-020-01732-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Extracellular protein production in bioreactors under fed-batch mode. a Comparison between RUT-C30 and the engineered Br_TrR03 strain in bioreactor cultivations with an inducer-rich medium. b Extracellular protein production by Br_TrR03 during bioreactor cultivations with a molasses-based batch medium supplemented with different nitrogen sources: ammonium sulfate only, yeast extract, YEPD-grown yeast (YEPD yeast) and molasses-grown yeast (MG-yeast). The feed comprised sugarcane molasses or acid-inverted molasses (inv. molasses). For comparison purposes, a fermentation with the strain Br_TrR02 using acid-inverted molasses and molasses-grown yeast is shown. Results are expressed as mean ± SD (n = 3)