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. 2020 May 15;11:503. doi: 10.3389/fgene.2020.00503

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Copyright © 2020 Shen, Li, Chen, Wu, Wang, Chen, Xie, Wang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental validation of circRNA. (A) PCR products were detected in agarose gel electrophoresis; (B) Sanger sequencing for the back-splice site of circRNA circulation; (C) RT-qPCR test for the expression level. Figures of small letter a, b, c and d represents 4 circRNAs and its host genes as following order: novel_circ_0013493(MINDY4), novel_circ_0007947(INPP4), novel_circ_0017683(CDC42BPA) and novel_circ_0018947(ESR1), respectively.