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. 2020 May 15;11:568. doi: 10.3389/fphar.2020.00568

Figure 4.

Figure 4

The effect of Tan IIA on producing pro-inflammatory cytokines and MMPs in RA-FLSs. (A) The effect of 0, 2.5, 5, 10, and 20μM Tan IIA on cell viability induced by TNF-α (20 ng/mL). (B) The effect of 10 μM and 20 μM Tan IIA on relative mRNA expression of pro-inflammatory cytokines induced by TNF-α (20 ng/mL) normalized with β-actin in RA-FLSs. (C) The effect of 10 μM and 20 μM Tan IIA on relative mRNA expression of MMP-2, MMP-3, MMP-8, and MMP-9 induced by TNF-α (20ng/mL) compared to β-actin in RA-FLSs. (D) The effect of Tan IIA on pro-inflammatory cytokines release induced by TNF-α in RA-FLSs. a. The effect of 10 μM and 20 μM Tan IIA on IL-6 release induced by TNF-α (20ng/mL). b. The effect of 10 μM and 20 μM Tan IIA on IL-1β release induced by TNF-α (20ng/mL). c. The effect of 10 μM and 20 μM Tan IIA on IL-8 release induced by TNF-α (20ng/mL). The values are the mean ± SEM from at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl (0 μM Tan IIA and 0 ng/mL TNF-α). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. group treated with TNF-α (20 ng/mL).