W-Acidic motif with the sequence LEWD conserved in vertebrate Nup358. Sequences of Nup358 from vertebrates were aligned. The sequence region homologous to human Nup358-min (residues 2147–2240) is shown. The location of the W-acidic motif is schematically indicated in a top panel. Residues are colored in different shades of purple, according to their degree of conservation (see the scale). Species names are abbreviated. Sequences from the following species are shown (in alphabetical order): Ailuropoda melanoleura, Anolis carolinensis, Astrofundulus limneaus, Bos taurus, Capra hircus, Castor canadensis, Equus caballus, Ficedula albicollis, Gopherus agassizii, Homo sapiens, Ictidomys tridecemilneatus, Lonchura striata domestica, Loxodonta africana, Macaca mulatta, Mandrillus leucophaeus, Mesocricetus auratus, Mus musculus, Myotis lucifugus, Ornithorhynchus anatinus, Oryctolagus cuniculus, Ovis aries, Pan troglodytes, Papio anubis, Patagioenas fasciata monilis, Rattus norvegicus, Salmo salar, Sus scrofa, Urgus americanus, and Xenopus tropicalis. The same sequence alignment is also shown for a larger region of Nup358 in Figure S2.