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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Biochemistry. 2019 Dec 6;58(50):5085–5097. doi: 10.1021/acs.biochem.9b00907

Figure 6.

Figure 6.

BicD2-CTD and KLC2 are capable of binding simultaneously to Nup358-min. (A–H) Purified BicD2-CTD, KLC2TPR, and Nup358-min were combined in an equimolar ratio and analyzed by gel filtration chromatography. SDS–PAGE gels of the elution fractions are shown. (A) Nup358-min and BicD2-CTD. (B) Nup358-min and KLC2TPR. (C) KLC2TPR, Nup358-min, and BicD2-CTD. (D and E) Same as panel C. 2x denotes a 2-fold molar excess of BicD2-CTD (D) or KLC2TPR (E). Samples in panels D and E were concentrated after mixing prior to analysis. (F) KLC2TPR. (G) Nup358-min. (H) BicD2-CTD. SDS–PAGE analyses of the purified proteins and of the loaded samples are shown in Figures S3 and S4, respectively. The experiments were repeated, and very similar results were obtained. The numbers of replicates for experiments shown in each figure panel were (A) 3, (B) 4, (C) 2, (D) 2, (E) 2, (F) 3, (G) 4, and (H) 5.