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. 2020 May 12;8:269. doi: 10.3389/fcell.2020.00269

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Copyright © 2020 Lin, Qu, Wang, Yang, Hu, Hu, Wu, Jiang, Liu and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The inhibition of ERK1/2 suppresses morphine-induced releasing of HSP70 and attenuates the ER stress and the phosphorylation of PKA and NR-1. Representative western blot images were shown in this figure. (A) Glibenclamide abolished the phosphorylation of ERK1/2 elevated by morphine. Glibenclamide (200 μM) was given 15 min before morphine (200 μM, 1 h) administration. Cell extracts were collected and analyzed by western blot (n = 3). (B) ERK1/2 inhibitor, SCH772984, could suppress the phosphorylation of ERK1/2 induced by morphine treatment in SH-SY5Y cells. SCH772984 (2 μM) was given 15 min before morphine (200 μM, 1 h) administration. Cells were collected 12 h after morphine treatment and analyzed by western blot (n = 3). (C) HSP70 release induced by morphine was reversed by ERK1/2 inhibitor in SH-SY5Y cells. SCH772984 (2 μM) was given 15 min before morphine (200 μM, 12 h) administration. Supernatants were collected and analyzed by western blot (n = 3). (D–H) Pretreatment with ERK1/2 inhibitor could inhibit the up-regulation of ER stress molecule GRP78, ATF6, IRE-1α, XBP1s and Caspase-12 induced by morphine in SH-SY5Y cells. SCH772984 (2 μM) was given 15 min before morphine (200 μM, 12 h) administration. Cells were collected 12 h after morphine treatment and analyzed by western blot (n = 3). (I) ERK1/2 inhibitor decreased the phosphorylation of PKA induced by morphine in SH-SY5Y cells. SCH772984 (2 μM) was given 15 min before morphine (200 μM, 12 h) administration. Cells were collected 12 h after morphine treatment and analyzed by western blot (n = 3). (J) Pretreatment with ERK1/2 inhibitor could inhibit the phosphorylation of NR-1 induced by morphine in SH-SY5Y cells. SCH772984 (2 μM) was given 15 min before morphine (200 μM, 12 h) administration. Cells were collected 12 h after morphine treatment and analyzed by western blot (n = 3). (A–J) data were analyzed by one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. control, ^#P < 0.05, ^##P < 0.01, ^###P < 0.01 vs. morphine-treated group).