Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 12;8:269. doi: 10.3389/fcell.2020.00269

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Lin, Qu, Wang, Yang, Hu, Hu, Wu, Jiang, Liu and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Overexpression of HSP70 can improve ER stress-induced by morphine in SH-SY5Y cells. Representative western blot images were shown in this figure. (A) Adenovirus-mediated HSP70 overexpression inhibited the decrease of intracellular HSP70 caused by morphine in SH-SY5Y cells. Cell extracts were collected 12 h after morphine treatment and analyzed by immunoblot assay (n = 3). (B–E) Adenovirus-mediated HSP70 rescue could inhibit the up-regulation of ER stress molecule GRP78, IRE1α, XBP1s and Caspase-12 induced by morphine in SH-SY5Y cells. Cells were collected 12 h after morphine treatment and analyzed by western blot (n = 3). (A–E) data were analyzed by one-way ANOVA (*P < 0.05, ***P < 0.001 vs. control, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. morphine-treated group).