Skip to main content
. 2020 Apr 3;69(6):1206–1218. doi: 10.2337/db19-0687

Figure 1.

Figure 1

Human intraislet macrophages share features of mouse islet stromal macrophages and rarely enter the islet parenchyma. A: Maximum projection of confocal images from a fixed-frozen pancreatic tissue section from a human showing CD206 (green) and Iba1 (red) immunostaining. DAPI (blue) shows nuclei. Scale bar, 50 μm. Islet border denoted by white dotted line. A’: Zoomed image of human macrophages in A, with Iba1+ (red) macrophages (*) corresponding to those inside of the islet border. A’’: Zoomed image of human macrophages in A, with CD206+ (green) macrophages (*) corresponding to those inside of the islet border. Scale bar, 20 μm, also applies to A’. B: Maximum projection of confocal images from a fixed-frozen C57BL/J6 mouse pancreatic tissue section showing CD206 (green), Iba1 (red), and insulin (white) immunostaining. DAPI (blue) shows nuclei. Scale bar, 50 μm. Islet border denoted by white dotted line. C: Quantification of the percentage of CD206+ macrophages found within the islet parenchyma in human and mouse islets (Intra) and in the peri-islet (Peri) area. D: Maximum projection of confocal images from a fixed-frozen human pancreatic tissue section showing macrophages labeled with Iba1 (red) and the vessel marker CD31 (cyan). Islet border denoted by white dotted line. Scale bar, 50 μm. E: Maximum projection of confocal images from a fixed-frozen C57BL/J6 mouse pancreatic tissue section showing macrophages labeled with Iba1 (red) and the vessel marker CD31 (cyan). Islet border denoted by white dotted line. Scale bar, 50 μm. Zoomed images of human macrophages (F) and mouse macrophages (G), with Iba1+ macrophages inside of the intraislet vessels (CD31). Scale bar, 25 μm. H: Quantification of the percentage of Iba1+ immunostaining found outside the islet CD31-labeled area in human and mouse peri-islet (Peri) and intraislet (Intra) regions. I and J: Maximum projection of confocal images from a fixed-frozen human pancreatic tissue section showing macrophages labeled with Iba1 (red) and the stromal marker PDGFRβ (cyan). Islet border denoted by white dotted line. Scale bar, 25 μm. I’: Zoomed image of human macrophages in I, with Iba1+ (red) macrophages inside of the islet stroma labeled for PDGFRβ (cyan). J: Maximum projection of confocal images from a fixed-frozen C57BL/J6 mouse pancreatic tissue section showing macrophages labeled with Iba1 (red) and the stromal marker PDGFRβ (cyan). Islet border denoted by white dotted line. Scale bar, 25 μm. K: Quantification of the percentage of Iba1+ immunostaining found outside the islet stroma (PDGFRβ-labeled) area in human and mouse peri-islet (Peri) and intraislet (Intra) areas. *P < 0.05, ANOVA.