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. 2020 May 21;8:71. doi: 10.1186/s40168-020-00850-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Genetic profile of the 438 significantly differentially abundant microbial genes between irrigation treatments. a Heatmap of genes enriched or depleted (FDR p < 0.01) in the metagenomes of TWW-irrigated roots (displaying trimmed mean of M values TMM). Gene abundance was normalized by scaling each row separately. The gene list was clustered to high hierarchy SEED categories. b The proportion of genes enriched or depleted in TWW-irrigated root metagenomes compared to the total abundance of that category in all metagenome analyses. Enriched category (TWW, magenta), deprived (FW, light blue), or the proportion within the full gene catalogue (marked as “all”, colored by orange), are highlighted. The proportion was calculated based on the number of genes assigned to the different categories with-in each data set