Fig. 4.

Chromatin organization of subtelomeric elements is independent of telomere length and the SIR complex. In vivo ChEC experiments with MN-RAP1 analyzed on Y’ elements in a, TEL03L in b and TEL06R in c in presence (YKU80) or in absence of the Yku complex (yku80Δ). In each panel, the left shows a Southern blot with digested genomic DNA hybridized with the appropriate specific probe. On the right is a schematic diagram with the averaged MN-Rap1-induced cutting sites ± standard deviation determined from at least 2 independent experiments plotted with respect to the XhoI site and other previously described features. d–f In vivo ChEC experiments with MN-RAP1 analyzed on Y’ elements in d, TEL03L in e and TEL06R in f in presence (SIR4) or in absence of the SIR complex (sir4Δ)