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. 2020 May 14;11:450. doi: 10.3389/fpls.2020.00450

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Copyright © 2020 Wang, Yang, Kong, Li, Li, Du and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Promoter analysis of GmPAP12 in transgenic soybean nodules. (A) Expression of GmPAP12 in nodules determined by qRT-PCR. (B) cis-Elements of GmPAP12 promoter analyzed by PlantCARE. (C) Expression of GmPHR1 in nodules determined by qRT-PCR. Nodules were harvested at 0, 10, 17, and 28 days post rhizobium inoculation in normal growth condition. (D) The diagram shows the construct of GmPAP12 promoter for yeast one-hybrid (Y1H). (E) Y1H results. Three tandem repeats of each P1BS element were used as baits and cloned into pAbAi vector. The resulting pAbAi-3xP1BS vectors were transformed into Y1H cells expressing PGADT7-AD (negative control) or PGADT7-AD-GmPHR1, respectively. The transformants were grown on selective medium SD-Leu containing antibiotic AbA (250 μg/ml), and pictures were taken after 4 days of incubation at 30°C. The Y1H assays were repeated three times. Letters in (A,C) indicate significant differences (one-way ANOVA, p < 0.05).