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. 2020 May 14;11:450. doi: 10.3389/fpls.2020.00450

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Copyright © 2020 Wang, Yang, Kong, Li, Li, Du and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Promoter analysis of GmPAP12 gene in transgenic soybean nodules. (A) Histochemical GUS staining of wild-type (WT) nodules growing in low P (LP) conditions. (B) Histochemical GUS staining of GmPAP12 promoter transgenic nodules growing in high P (HP) conditions. (C) Histochemical GUS staining of GmPAP12 promoter in transgenic nodules growing in LP conditions. Promoter of GmPAP12-GUS transgenic composite soybean plants was treated with different P levels, and nodules were harvested 28 days after inoculation with rhizobia for GUS staining. (D) GUS activity of GmPAP12 promoter in transgenic nodules. Values are means of 10 independent lines for each P treatment. Bars show the means ± SD values. Asterisks indicate significant difference within a P level in t-tests. **p < 0.01.