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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cell Tissue Res. 2019 Jun 14;378(1):15–32. doi: 10.1007/s00441-019-03050-6

Fig. 5. Cochleotopic projections reveal profound differences at E18.5 in all six Npr2 mutants studied.

Fig. 5

Triple dye injections into the vestibular organs (NVJ, lilac), apex of the cochlea (NVM, green) and base of the cochlea (NVR, red) show profound afferent distribution differences between control (a,a’,a”) and Npr2 null mutants (b,b’,b”) but show no effect on overall cochleotopic dorsoventral patterning. Most notable were profoundly reduced basal projections to the AVCN (a,b,a”,b”) and profoundly expanded projections of apex to the PVCN and DCN (a’,b’). Measuring the extension of afferents showed a significant reduction in the length of apical or basal cochlear projections in Npr2 ko mice to the AVCN (Table 1). NVJ is excited at 488nm and thus results in background fluorescence that was used to outline the cochlear nuclei (a) and also labels the choroid plexus (CP in a). Bar indicates 100 μm.