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. 2020 May 15;11:485. doi: 10.3389/fphys.2020.00485

FIGURE 6.

FIGURE 6

(A) The expression level of Lv-VgR mRNA at 0, 24, and 48 h after the injection of PBS, NC-siRNA or VgR-siRNA, and different lowercase letter represented a significant level (P < 0.001, ANOVA followed by Fisher’s LSD test). In this study, the data are expressed as mean ± SE (n = 6). (B) The gonadosomatic index (GSI) of female shrimp at 0, 24, and 48 h after the injection of PBS, NC-siRNA or VgR-siRNA. Different ovarian developmental stages were represented by I, II, III, and IV, respectively. The significant differences were assessed using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001). In this study, the data are expressed as mean ± SE (n = 4). (C) Morphological and anatomical analysis for the ovarian development of L. vannamei. Panels (A,C,E,G,I,K,M) are dorsal views. Panels (B,D,F,H,J,L,N) are lateral views. Panels (O–U) are the ovaries obtained from the shrimp above. Scale bars: 2.5 cm. (D) Sections of ovaries stained with hematoxylin and eosin. Panels (A,B) are uninjected controls, (C–F) saline, (G–J) NC-siRNA, (K–N) VgR-siRNA injected treatments, (O–T) are the enlarged regions above. Numerals I–IV refer to the developmental stages of the oocytes. Scale bars: 400 μm, inserts 8 μm.