TABLE 1.

Primers used for cloning, RT-PCR, DIG-labeled DNA probe and the sequences for siRNA synthesis.

Primer name	Sequence (5′–3′)	Purpose
VgR-5′end-F VgR-5′end-R VgR-A-F VgR-A-R	GGAAGAAGCTCCTCCCAAGA AGTGGACGCAGTCTGTAACCT TGCGACAACGACGTGGATT TGTCGTTACACTTGACGGATTCAC	Primer for 5′ end fragment Primer for 5′ end fragment Middle fragment A Middle fragment A
VgR-B-F VgR-B-R VgR-C-F VgR-C-R	GTTCGCAAATCTTGTGGTCTGA CGGCATAAGGGCTTTCGTC TCACCTGCGGCAACAAGA CCCACCGTTTCCCAAGC	Middle fragment B Middle fragment B Middle fragment C Middle fragment C
VgR-D-F VgR-D-R VgR-3′race-F1 VgR-3′race-F2 VgR-3′race-F3 VgR-all-F VgR-all-R1	CTGACGAAAGCCCTTATGCCG TCGGCTGTGCTCACTATGGC TTCCTTGGACCCCGTTAGCA TTCTGTGATAATGGTCTGCGGGATG ATTGGACTAGCCCTGAGTGGAG GGAAGAAGCTCCTCCCAAGA TGCGTGCTTCACAAAACAGTTCA	Middle fragment D Middle fragment D 3′ RACE 3′ RACE 3′ RACE Full length Full length
VgR-all-R2 VgR-q-F VgR-q-R GAPDH-F GAPDH-R VgR-PF VgR-PR VgR-831-F VgR-831-R EGFP-482-F EGFP-482-R	CCACATTGATACCTGGATTTTACC TGGTTCTCCTCGTCTTGGCTCTG GGTGGTGGCGTTCGTGGTTG CCCTTCATCACGCTGGACTAC AACACACCAGTGGACTCAACGA TCACCTGCGGCAACAAGA CCCACCGTTTCCCAAGC GCGAAUCCCAAGACCAUUATT UAAUGGUCUUGGGAUUCGCTT GCAUCAAGGUGAACUUCAA UUGAAGUUCACCUUGAUGC	Full length RT-PCR RT-PCR RT-PCR RT-PCR Synthesis of DIG-labeled DNA probe Synthesis of DIG labeled DNA probe VgR siRNA VgR siRNA NC siRNA NC siRNA