FIGURE 3.

FA synthesis pathway of B. subtilis illustrating the observed changes in protein expression in response to both cerulenin addition and rescue of cellular growth with exogenous FA supplementation using n16:0 and a15:0 FAs. Cerulenin irreversibly binds to FabF, blocking FA synthesis. The observed 6.6-fold increase in FabF is consistent with previous reports (Schujman et al., 2001). These results also indicate that many other proteins in the pathway are overexpressed in response to these conditions, including the regulation protein, FapR. For locus tag, UniProt Description, log2 change in expression, and p-values for each differentially expressed protein (see Supplementary Table S2).