FIGURE 4.

FA degradation pathway of B. subtilis illustrating the observed changes in protein expression in response to both cerulenin addition and rescue of cellular growth with exogenous FA supplementation (n16:0 and a15:0). The ΔyusL deletion has removed FadN in both cases, yet there appears to be an increase in the amount of enzymes preceding the deletion when the cells have been subjected to the FA labeling strategy. For locus tag, UniProt Description, log2 change in expression, and p-values for each differentially expressed protein (see Supplementary Table S2).